This plasticity is the result of the existence of very efficient input pathways that convey the external signals into the core oscillator machinery. Main Research Areas (click to expand headings below for more information). We have adapted and optimized the new CRISPR-based technology, prime editing, to generate precise changes into a target genomic location. UAS-gypsy structure and functional test. Simchovitz A, Hanan M, Yayon N, Lee S, Bennett ER, Greenberg DS, Kadener S, Soreq H. A Parkinson's disease CircRNAs Resource reveals a link between circSLC8A1 and oxidative stress. miR-184regulates pancreatic -Cell function according to glucose metabolism. Second, we identified 53 transcription factors, specifically expressed or enriched in the ISCs, and are characterizing how they cooperate to integrate signal inputs. The Perrimon lab is interested in characterizing the mechanisms by which cells, tissues and organs communicate during development and in mature animals. Selected for Faculty of 1000 (Factor 3.0 Recommended). Jens joined the Bando Lab in 2023 as a visiting graduate student to understand how distinct histone mutations influence gliomagenesis and also aims to exploit the dependence of pLGGs on the MAPK pathway. Simchovitz A, Hanan M, Yayon N, Lee S, Bennett ER, Greenberg DS, Kadener S, Soreq H. A Parkinson's disease CircRNAs Resource reveals a link between circSLC8A1 and oxidative stress. and Torres H.N. . Gut tumors can be induced in adult flies by overexpressing an active form of the transcriptional co-activator yorkie/YAP using the intestinal stem cell driver escargot-GAL4. Adam then moved to Boston for his postdoc in the lab of Dr. Yang Shi where he grew interested in identifying epigenetic targets for pediatric brain cancers. SRCP: a comprehensive pipeline for accurate annotation and quantification of circRNAs. This plasticity is the result of the existence of very efficient input pathways that convey the external signals into the core oscillator machinery. Associate Professor, Brandeis University. In mature animals, cells and organs interact to maintain homeostasis and coordinate their physiological roles. What is the contribution of non-coding RNAs (small and large) to the robustness of the circadian system? Adam is currently studying approaches to therapeutically target cancers that harbor mutations in the Mismatch Repair pathway. Sayan is a graduate student in the Molecular and Cell Biology program at Brandeis and he is investigating the role of circRNA in aging and neurodegeneration. Genome-Wide Analysis of Light- and Temperature-Entrained . Dec 2017. Hanan M., Soreq H. and Kadener S. CircRNAs in the brain. What are the key functions of these molecules in the brain and during aging? and Kornblihtt A.R. Articles Cited by Public access Co-authors. The EMBO journal 20 (20), 5759-5768, 2001. . Regulation of RNA metabolism is emerging as a major regulatory hub for gene expression control in tissues like the brain and the muscle, which don't undergo cel. The challenge is now to understand how these pathways intersect and cooperate, as it is not clear if some pathways regulate the activity of others, or if they act concomitantly. 502. In addition and most importantly, genome-wide libraries of transgenic RNAi lines are available that allow knockdown of any. Fun fact:Riley chose her English name when she moved to the U.S because it means courage. We also identified the first factor involved in circRNA production, the splicing factormuscleblind. He completed his internship and pediatric residency training at Childrens National Medical Center, before immersing himself in pediatric hematology/oncology training followed by subspecialty Pediatric Neuro-Oncology training at Childrens Hospital Los Angeles/University of Southern California. She is the Co-Director of Research for a non-profit, the STEM Advocacy Institute, which is focused on building a future where there is equitable access and exposure to science for all people. I am an undergraduate student in the class of 2024 majoring in Neuroscience and HSSP. 1, Artyomov M., Shishkin A., Kadri S., Zhu X., Gutman M., Garber M.* and Kadener S.* Defining the 5 and 3 landscape of theDrosophilatranscriptome with ExoCAGE and RNaseH-seq. To address this need, we have shown that two NanoTags, VHH05- and 127D01-tagsthat are 14 and 10 amino acids in length, and their corresponding nanobodies (NbVHH05 and Nb127D01) are excellent reagents for both in vitro and in vivo studies in Drosophila. J. Biol. Tattikota SG., Rathjen T., Hausser J., Khedkar A., Kabra UD., Pandey V., Sury M., Wessels HH., Mollet IG., Eliasson L., Selbach M., Zinzen RP., Zavolan M., Kadener S, Tschp MH, Jastroch M, Friedlnder MR., Poy MN. at USC we have extended this approach to the mouse. Rosi is accepted to attend the Autumn Workshop in Structural Proteomics in Nurmes, Finland and was chosen to receive a COST scholarship. Sebastian Kadener. Fun fact: Michelle is a huge Stephen King fan. What are the key functions of these molecules in the brain and during aging? CRISPR-generated mutant cell lines, in combination with RNAi, provide a robust platform for combinatorial screening. Circadian clocks are remarkably robust: they are able to keep time without timing cues and are resilient to large variations in environmental conditions. Transcriptional activators differ in their abilities to control alternative splicing. This work, led by Sebastian Kadener, summarizes years of development of a pipeline that improves upon current tools. Cramer P., Cceres J.F., Cazalla D., Kadener S., Muro, A., Baralle F. and Kornblihtt A. Alexander M. van der Linden, Matthew Beverly, Sebastian Kadener, Joseph Rodriguez, Sara Wasserman, Michael Rosbash, Piali Sengupta. tested regenerative limb interventions to restore form and function to the amputated hindlimbs in adult African clawed frogs. Fun fact: Outside the lab, Adam is an avid soccer fan and enjoys watching the English Premier League. * Corresponding authors. Tattikota SG., Rathjen T., Hausser J., Khedkar A., Kabra UD., Pandey V., Sury M., Wessels HH., Mollet IG., Eliasson L., Selbach M., Zinzen RP., Zavolan M., Kadener S, Tschp MH, Jastroch M, Friedlnder MR., Poy MN. *, Eliaz D.*, Cohen Chalamish S., Tschudi C., Unger R., Hashem Y., Kadener S. and Michaeli S. Pseudouridylation in. Portal D., Lobo G.S., Kadener S., Prasad J., Espinosa J.M., Pereira C.A., Tang Z., Lin R.J., Manley J.L., Kornblihtt A.R., Flawia M.M. Control of alternative pre-mRNA splicing by RNA Pol II elongation: faster is not always better. Jens is co-mentored by Dr. David Jones at the DKFZ. Our major contributions to tool development are the Gal4-UAS system, FLP-FRT germline clone technique, in vitro and in vivo genome-wide RNAi and CRISPR screening platforms and resources, and in vivo proximity labeling methods. He is returning to Brandeis as an Associate Professor of Biology. Sebastian Kadener Prof. Nac. He is now very excited to use his skills to contribute to cancer research, specifically focusing on improving our understanding of childhood brain tumors and the development of more effective treatments. Bartok O., Teesalu M., Pandey V., Hanan M., Poukkula M., Havula E., Moussaieff A., Vodala S., Nahmias Y., Kadener S.* and Hietakangas V.* Cabut-dependent repressive branch of the sugar sensing transcriptional network regulates glyceroneogenesis. In a collaboration with Sebastian Kadeners lab at Brandeis, we generated a new set of PspCas13b and RfxCas13d expression constructs that can be used to target RNA in cells and in vivo. We showed that their translation is mediated by IRES-like sequences, is enhanced in response to starvation and happens in association with membranes and in synapses. The EMBO journal 20 (20), 5759-5768, 2001. We believe that this is reminiscent of changes in ISC proliferation observed in animals that feed infrequently, such as the Burmese python, where ISC proliferation is triggered following a large meal as a result of gut distortion. Recent reports revealed that circRNAs are expressed in developmental- and tissue-specific manners and are highly enriched in the nervous system, particularly in synapses. We showed that miRNAs have a key role in providing robustness to the circadian system both during development and in adults. She then moved from the Bay Area to Boston where she completed her Pediatrics residency at the Boston Combined Residency Program at Boston Childrens Hospital and Boston Medical Center in 2018, before joining the Dana-Farber/Boston Childrens Cancer and Blood Disorders Center as a Pediatric Hematology/Oncology/Stem Cell Transplant Fellow. single nuclei sequencing (snRNAseq) of entire flies, and proteomics approaches, e.g. Control of alternative pre-mRNA splicing by RNA Pol II elongation: faster is not always better. We also identified the first factor involved in circRNA production, the splicing factormuscleblind. We are currently focusing on transcription factors with mammalian orthologs implicated in epithelial homeostasis or cancer, and are using targeted DamID (TADA) to characterize their targets. Weiss R., Bartok O., Mezan S., Malka Y and Kadener S. Synergistic Interactions between the Molecular and Neuronal Circadian Networks Drive Behavioral Circadian Rhythms in. Mezan S., Feuz JD, Deplancke B. and Kadener S. PDF signaling is an integral part of the. Interestingly, circRNAs accumulate in an age-dependent manner suggesting their relevance to age-related homeostasis and/or pathogenesis. Cs. miR-184regulates pancreatic -Cell function according to glucose metabolism. During development, cells have to interact to coordinate proliferation and differentiation to form tissues and organs. For her Masters thesis, she studied the role of thermosensitive splicing isoforms of the circadian genetimeless on the circadian rhythm in Drospholia. leptin), neural development, and behavior are conserved. Aaron received his medical degree from Thomas Jefferson University in 2011. Now, in an article published in the journal Molecular Cell, the lab of Dr. Sebastian Kadener at the Hebrew University of Jerusalem, in collaboration with the lab of Prof. Nikolaus Rajewsky at the . My diverse. cabut during early embryogenesis, dorsal closure and nervous system development. Bartok O., Kyriacou C., Levine J., Sehgal A. and Kadener S. Adaptation of molecular circadian clockwork to environmental changes: a role for alternative splicing and miRNAs. I am interested in studying circular RNAs and neurological disorders. He also enjoys playing handball and online chess. Circadian clocks are remarkably robust: they are able to keep time without timing cues and are resilient to large variations in environmental conditions. Samar was awarded the Neubauer doctoral fellowship . These tools, which are available viaDRSC/TRiP Functional Genomics Resources, include: DIOPTDIOPT-DISTGene2FunctionGLADBioLitMineUP-TORR, RSVPSnapDragonFlyPrimerBankFind CRISPRSNP-CRISPRDGET. Jess hopes to pursue a career as a physician-scientist, taking care of children with brain tumors and other solid tumors and working hard to improve treatments for her patients. Fun fact: In his native language of Urdu, Shers full name translates to lion/tiger brave. There, she studied the oncogenic role of certain signaling molecules in the Hedgehog signaling pathway, and became extremely interested in advancing cancer therapy research. Kadener S., Fededa J.P., Rosbash M. and Kornblihtt A.R. 0. 369. Nucleic Acids Research47(11): e95; 1-16 (2017). Community support Reprint. Informative set of videos on insect physiology and endocrinology: New paper: What are the neural and molecular basis of temperature adaptation and compensation? He is a Neuroscience graduate student investigating the molecular mechanisms of the splicing factor muscleblind in the Drosophila nervous system. Jess received her BS in Biological Sciences with Honors in Neurobiology at Stanford University in 2008 before joining the Medical Scientist Training Program at Stanford University School of Medicine, receiving her MD and PhD in 2015. These two short peptide tags and their nanobodies can be used for labeling and manipulating proteins. Zaffagni M, Kadener S. NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress. He plans to continue his career as a physician-scientist in pediatric oncology. Contact the author . The Kadener laboratory studies how molecular processes in the brain determines behavior with a special emphasis on RNA metabolism. Our Funds. For example, we showed that circRNA biogenesis competes with canonical splicing, showing that circRNAs can function incisas RNA traps. RNA biology Neurosciences. RNAseq of specific organs and more recently, whole-body snRNAseq, allow us to define the transcriptional signatures corresponding to homeostatic states, various stress conditions, and changes to the diet and environment. High-accuracy determination of internal circadian time from a single blood sample. Third, we found that the stretch activated calcium channel Piezzo regulates ISC proliferation and differentiation. Zaffagni M, Kadener S. NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress. Explore trending content on Musk Viewer For his senior clinic project, John worked with HRL Laboratories to create an algorithm for electrostatically tuning quantum dots using neural networks for quantum computers. Selected for Faculty of 1000 (Factor 3 Recommended). Mouse-olfactory bulb, brain stem, and thalamus-Mock. As an underlying mechanism, the balance or critical reaction hypothesis have been proposed. He can also be found doing crossword puzzles at odd hours. Stem cell fate is determined by the interplay of lineage-specific intrinsic factors and extrinsic signals, acting primarily at the transcriptional level. Cell fate is determined by the interplay of lineage-specific intrinsic factors and extrinsic,! S. PDF signaling is an avid soccer fan and enjoys watching the English Premier League cell... 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